Shaw Nature Reserve is on I-44 in Gray Summit, Missouri, approximately 32 miles southwest of the Missouri Botanical Garden (in St. Louis) and 22 miles west of the I-44/I-270 interchange. The Gray Summit I-44 exit is #253, just two exits west of Six Flags (Allenton/Eureka).
- Westbound I-44 from exit ramp, turn left, crossing over I-44. Turn right at the intersection (at the Mobil) and go 50 yards to the large iron gates on the left side of the road.
- Eastbound I-44, from exit ramp #253, turn right at top of ramp and then another right at at intersection (at the Mobil) and go 50 yards to the large iron gates on the left side of the road.